Year: 1974
Type: article
Abstract: The degradation of cellular proteins in fibroblasts, both those of rapid and those of slow turnover rates, was inhibited by low concentrations of chloroquine or neutral red in the medium. Cells inhibited by chloroquine can be inhibited further by fluoride. Chloroquine was taken up by the fibroblasts and the concentration in the cells reached several hundred times that in the medium. Isopycnic fractionation studies showed that within the cells the chloroquine was concentrated in the lysosomes, and that these chloroquine-containing lysosomes had a lower equilibrium density than the lysosomes of untreated cells. Chloroquine, at concentrations attained inside the lysosomes, inhibited cathepsin B(1) but not cathepsin D. It is concluded that chloroquine impairs the breakdown of cellular proteins after these have entered the lysosome system, probably through inhibition of cathepsin B(1).
Source: The Journal of Cell Biology
DOI: 10.1083/jcb.63.2.430
Authors: Maurice Wibo, Brian Poole
Institution: Rockefeller University
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: green
Funder: U.S. Public Health Service
Award: G7052392956
OpenAlex ID: W2095894333