Quantification of a New Anti-Cancer Molecule MJC13 Using a Rapid, Sensitive, and Reliable Liquid Chromatography-Tandem Mass Spectrometry Method.
Year: 2015
Type: article
Abstract: 392 for IS. The retention times for MJC13 and IS were 4.98 min and 4.42 min, respectively. The standard curves of MJC13 in solution, rat plasma, and rat urine were linear in the concentration range of 1 - 1000, 1 - 1000 and 1 - 200 ng/mL, respectively. The intra- and inter-day accuracy (relative error) ranged from 1.99 - 4.20% and 1.83 - 4.39%, respectively. The intra- and inter-day precision (coefficient of variation) ranged from 2.27 - 3.88% and 2.80 - 4.79%, respectively. The extraction recovery rates of rat plasma and urine samples were 95.3% and 96.2%, respectively. No measurable matrix effect interfered with MJC13 identification and quantification in rat plasma and urine. In summary, a rapid, specific, sensitive, and reproducible LC-MS/MS method was developed and validated to quantify MJC13 in solution, rat plasma, and rat urine.
Source: PubMed
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: Functional Characterization of FKBP52 Interactions with Steroid Hormone Receptors, RCMI Translational Research Network (RTRN), Border Biomedical Research Center, Drew RCMI Translational Research Network, Development of a Novel Nanoconstruct Based Photothermal Therapy for Tumor Hypoxia, Border Biomedical Research Center, Institute for Biomedical and Health Disparities Research, Center for Biomedical and Health Research Excellence (CBHRE)
OpenAlex ID: W2096709243