Work

Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping

Year: 1991

Type: article

Abstract: Polymorphisms in genomic fingerprints generated by arbitrarily primed PCR (AP-PCR) can distinguish between strains of almost any organism. We applied the technique to the mouse (Mus musculus). The characteristic differences in the AP-PCR genomic fingerprints between strains will be of value in strain identification and verification. Using one primer, we genetically mapped four polymorphisms in a set of C57BL/6J x DBA/2J recombinant inbreds. One of these polymorphisms is a length variant. The method will allow rapid genetic mapping of DNA polymorphisms without Southern blotting.

Source: Nucleic Acids Research

DOI: 10.1093/nar/19.2.303

Authors: John Welsh, Charles Petersen, Michael McClelland

Language: English

Topic: Genetic Mapping and Diversity in Plants and Animals

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2096822099

FWCI: 20.04

Cites: 14

Cited by: 354

Related to: 9