Year: 1979
Type: article
Abstract: The autofluorescence other than intrinsic protein emission of viable cultured mammalian cells has been investigated. The fluorescence was found to originate in discrete cytoplasmic vesicle-like regions and to be absent from the nucleus. Excitation and emission spectra of viable cells revealed at least two distinct fluorescent species. Comparison of cell spectra with spectra of known cellular metabolites suggested that most, if not all, of the fluorescence arises from intracellular nicotinamide adenine dinucleotide (NADH) and riboflavin and flavin coenzymes. Various changes in culture conditions did not affect the observed autofluorescence intensity. A multiparameter flow system (MACCS) was used to compare the fluorescence intensities of numerous cultured mammalian cells.
DOI: 10.1177/27.1.220325
Author: J E Aubin
Institution: Max Planck Institute for Biophysical Chemistry
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: bronze
Funder: Medical Research Council Canada
OpenAlex ID: W2097053416