Work

The zinc-finger proto-oncogene Gfi-1b is essential for development of the erythroid and megakaryocytic lineages

Year: 2002

Type: preprint

Abstract: Gfi-1 and Gfi-1b are novel proto-oncogenes identified by retroviral insertional mutagenesis. By gene targeting, we establish that Gfi-1b is required for the development of two related blood lineages, erythroid and megakaryocytic, in mice. Gfi-1b(-/-) embryonic stem cells fail to contribute to red cells of adult chimeras. Gfi-1b(-/-) embryos exhibit delayed maturation of primitive erythrocytes and subsequently die with failure to produce definitive enucleated erythrocytes. The fetal liver of mutant mice contains erythroid and megakaryocytic precursors arrested in their development. Myelopoiesis is normal. Therefore, Gfi-1b is an essential transcriptional regulator of erythroid and megakaryocyte development.

Source: Genes & Development

DOI: 10.1101/gad.959102

Authors: Shireen Saleque, Scott Cameron, Stuart H. Orkin

Institutions: Howard Hughes Medical Institute, Harvard University, Dana-Farber Cancer Institute, Massachusetts Institute of Technology

Language: English

Topic: Hematopoietic Stem Cell Transplantation

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Howard Hughes Medical Institute