Improvement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system
Year: 2010
Type: article
Abstract: Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out during 24 h of incubation of OPH-displaying cells at 30 degrees C. p-Nitrophenol produced in the reaction mixture was detected by HPLC. The strain with highest activity showed 8-fold greater OPH activity compared with cells engineered using glycosylphosphatidylinositol anchor system, and showed 20-fold greater activity than Escherichia coli using the ice nucleation protein anchor system. These results indicate that Flo1p anchor system is suitable for display of OPH in the cell surface-expression systems.
Source: Biotechnology Letters
Authors: Takeshi Fukuda, Kouta Tsuchiyama, Hirokazu Makishima, Katsumi Takayama, Ashok Mulchandani, Kouichi Kuroda, Mitsuyoshi Ueda, Shin-ichiro Suye
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,890
OpenAlex ID: W2097436638