Work

Transcription Analysis of Class II Human Leukocyte Antigen Genes from Normal and Immunodeficient Β Lymphocytes, Using Polymerase Chain Reaction

Year: 1990

Type: article

Abstract: The RNA transcript levels of all human leukocyte antigen class II loci were determined from class II congenital immunodeficient B cells by polymerase chain reaction amplification of cDNA. No mRNA was observed under conditions in which 0.01% normal levels could be visualized. Pre-mRNA could be amplified from normal B cells but not from immunodeficient B cells, indicating a transcription defect.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.10.7.3792-3796.1990

Authors: Michael Bull, Angeline Van Hoef, Jack Gorski

Language: English

Topic: Immunodeficiency and Autoimmune Disorders

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Institute of Allergy and Infectious Diseases

Awards: G1246138111, Molecular & Cellular Mechanisms in Transfusion Medicine, FUNCTION OF HLA-DR POLYMORPHISMS STUDIED BY MUTAGENESIS