Work

Novel Function of Rad27 (FEN-1) in Restricting Short-Sequence Recombination

Year: 2001

Type: article

Abstract: Saccharomyces cerevisiae mutants lacking the structure-specific nuclease Rad27 display an enhancement in recombination that increases as sequence length decreases, suggesting that Rad27 preferentially restricts recombination between short sequences. Since wild-type alleles of both RAD27 and its human homologue FEN1 complement the elevated short-sequence recombination (SSR) phenotype of a rad27-null mutant, this function may be conserved from yeast to humans. Furthermore, mutant Rad27 and FEN-1 enzymes with partial flap endonuclease activity but without nick-specific exonuclease activity partially complement the SSR phenotype of the rad27-null mutant. This suggests that the endonuclease activity of Rad27 (FEN-1) plays a role in limiting recombination between short sequences in eukaryotic cells.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.21.7.2349-2358.2001

Authors: M. Cristina Negritto, Junzhuan Qiu, Dawn O. Ratay, Binghui Shen, Adam M. Bailis

Institutions: Beckman Research Institute, City Of Hope National Medical Center

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green