Partial characterization of a bacterial acyltransferase enzyme for potential application in dairy processing
Year: 2014
Type: dissertation
Abstract: This study describes: the evaluation of the current, and potential assay methods for the quantification of cholesterol, cholesteryl esters and free fatty acids in milk and the application thereof; an account of the difficulties associated with the usage of FoodPro ® Cleanline, an enzyme preparation used as processing aid, during ultra-high temperature processing of milk; the development of activity assays which can be used for the kinetic characterization of glycerophospholipid cholesterol acyltransferase, the active enzyme in FoodPro ® Cleanline; the development of an accurate and facile activity assay, and the validation thereof, which can be used for the validation of enzyme activity prior to dosage of milk with FoodPro ® Cleanline.Prof P. Swart for always being available when needed, but giving me the freedom to think for myself, Tertius Cilliers for your enthusiastic approach to this project, and for trusting a "nat agter die ore" student with this work, Dr. Karl-Heinz Storbeck for always having an open door, even when I had senseless questions, Prof A. Swart for always being cheerful and open to any questions, Prof M. Rautenbach for all her help with the preparation of liposomes and small unilamellar vesicles during this study, Ralie Louw for running both the Water and P450 labs with the greatest enthusiasm and for her support and technical assistance, The members of the Water and P450 labs in no particular order: Timo for all the chats about everything and anything, Jonathan for always making me see the light side of everything, Cheryl for making work a cheerful experience, Terina for being the fairy lab mother, Lindie for your early morning hugs and support with the writing of this thesis, Liesl for always being open to anything no matter what, Barry for help with technical problems and Craig for teaching me how to think as a scientist and expect surprises, even if its missing proteins.You turned the lows of this project into highs, Kerneels Botha for allowing me to treasure hunt in your office when I need strange equipment, DuPont ® for financial support, Alex Zabbia and Peter Lawson for running the pilot scale trials, Helga, my fiancé, for always listening to my, often one-ended, conversations about work and for supporting me throughout this study, My brother Don and sister Carien, for your help, encouragement and support, Oom Theuns, Tannie Margeret, Theuns, Stephan and Tania Botha for all your teachings, support and for being my second family,
Author: Stefan Hayward
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W2098064604