Year: 2013
Type: article
Abstract: Progressive Multifocal Leukoencephalopathy (PML) is an often fatal disease caused by the reactivation of the JC virus (JCV). Better understanding of viral-host interactions has been hampered by the lack of an animal model. Engrafting NOD/SCID/IL-2-Rg (null) mice with human lymphocytes and thymus, we generated a novel animal model for JCV infection. Mice were inoculated with either a PML isolate, JCV Mad-4, or with JCV CY, found in the kidney and urine of healthy individuals. While mice remained asymptomatic following inoculation, JCV DNA was occasionally detected in both the blood and the urine compartments. Mice generated both humoral and cellular immune responses against JCV. Expressions of immune exhaustion marker, PD-1, on lymphocytes were consistent with response to infection. Using this model we present the first in vivo demonstration of virological and immunological differences between JCV Mad-4 and CY. This model may prove valuable for studying JCV host immune responses.
Source: PLoS ONE
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,382
Awards: Pathogenesis of a JC Virus Variant in Pyramidal Neurons, G1841839514, G2325943326, Cellular Immunity to JC Virus in AIDS-Associated Progressive Multifocal Leukoence, G453267231, G4700852728, Role of Inflammation in Progressive Multifocal Leukoencephalopathy, Translational applications of Progressive Multifocal Leukoencephalopathy studies, G7904766472, G8004881717, G8739412480, Harvard University Center for AIDS Research, Infectious Disease and Basic Microbiological Mechanisms, JC Virus Primary Infection, Latency, and Reactivation
OpenAlex ID: W2098162697