rsEGFP2 enables fast RESOLFT nanoscopy of living cells
Year: 2012
Type: article
Abstract: The super-resolution microscopy called RESOLFT relying on fluorophore switching between longlived states, stands out by its coordinate-targeted sequential sample interrogation using low light levels. While RESOLFT has been shown to discern nanostructures in living cells, the reversibly photoswitchable green fluorescent protein (rsEGFP) employed in these experiments was switched rather slowly and recording lasted tens of minutes. We now report on the generation of rsEGFP2 providing faster switching and the use of this protein to demonstrate 25-250 times faster recordings.DOI:http://dx.doi.org/10.7554/eLife.00248.001.
Source: eLife
DOI: 10.7554/elife.00248
Authors: Tim Grotjohann, Ilaria Testa, Matthias Reuss, Tanja Brakemann, Christian Eggeling, Stefan W Hell, Stefan Jakobs
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,500
Funder: Deutsche Forschungsgemeinschaft
Award: G392462602
OpenAlex ID: W2098275652