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Enzyme Activities in Chick Embryonic Cartilage

Year: 1971

Type: article

Abstract: Some characteristic enzymatic activities were determined in chick embryonic cartilage and compared with the analogous activities in bone and liver. Chondrocytes were isolated, broken by sonication, and subjected to subcellular fractionation to yield a nuclear pellet, the mitochondrial, lysosomal, and microsomal fractions, and the high speed supernatant solution. It was established that these fractions are characterized by enzymatic activities usually associated with similar fractions in other tissues, but with some quantitative differences. Lysozyme, a particulate-associated enzyme in other tissues, was not detected in any subcellular fraction even by the sensitive technique of microzone electrophoresis and is therefore considered to be primarily extracellular in cartilage.

Source: The Journal of Cell Biology

DOI: 10.1083/jcb.49.2.459

Authors: C. Arsenis, R. Eisenstein, L. W. Soble, K. E. Kuettner

Institutions: Rush University Medical Center, University of Illinois Chicago

Language: English

Topic: Osteoarthritis Treatment and Mechanisms

Subfield: Rheumatology

Field: Medicine

Domain: Health Sciences

Open Access status: green

OpenAlex ID: W2098735081

FWCI: 5.662

Cites: 1

Cited by: 24

Related to: 10