Protease-activated receptor-2 (PAR-2) regulates enterotoxigenic Escherichia coli-induced diarrhea during weaning in piglets
Year: 2010
Type: article
Abstract: Protease-activated receptor-2 (PAR-2) is a member of the G-protein-coupled receptor family. Theproteases that activate PAR-2 are released during inflammation and injury, with PAR-2 regulating theresponse to these insults. In the gastrointestinal tract, PAR-2 is known to alter gastrointestinalsecretion, motility, inflammation, and pain. In many cases, PAR-2 has been reported as proinflammatory and proliferative. Paradoxically, PAR-2 is also suggested to be anti-inflammatory in someinstances. Weaning piglet diarrhea is severely detrimental to the porcine industry, being responsible for11% of total piglet deaths, while those that survive from the disease experience developmentalproblems and fail to grow to the size of their healthy counterparts. Thus, we sought to determine anycorrelation between PAR-2 and weaning diarrhea. We hypothesized that PAR-2 might represent a newtarget in the treatment of weaning diarrhea. The current study measured PAR-2 usingimmunohistochemistry on sections of piglet gastrointestinal tract mucosa and identified changes inreceptor expression during the development and course of weaning. Moreover, the effect of PAR-2stimulation using lipopolysaccharide (LPS) and heat-labile enterotoxin (LT) on IL-6 and IL-8 productionin pig intestinal epithelial cells (IEC) was determined. This study found that PAR-2 is expressedabundantly in the piglet gastrointestinal tract mucosa, and revealed that PAR-2 mRNA and proteinexpression are both correlated with the severity of diarrhea. The generation of IL-6 and IL-8 by IECs wassignificantly increased following stimulation with PAR-2 agonists dose-dependently. Thus, we suggestPAR-2 may be involved in the development of diarrhea during weaning in piglets.
Source: African Journal of Pig Farming
Authors: Pei XiaoYing, Guo DingZong, Zhou DongHai, Rui Guo, Gao HongMei, Tian Xia
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2099255850