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PCR for Specific Detection of H7 Flagellar Variant of fliC among Extraintestinal Pathogenic Escherichia coli

Year: 2001

Type: article

Abstract: A newly developed PCR-based assay for the H7 variant of the Escherichia coli flagellin gene, fliC, was 100% sensitive and specific in comparison with serology and probe hybridization. It revealed broad conservation of the H7 fliC variant among phylogenetically diverse lineages of extraintestinal pathogenic E. coli (ExPEC) and superseded serotyping for certain isolates with ambiguous or non-H7 serotyping results. The H7 primers functioned well when incorporated into a multiplex PCR assay for diverse virulence-associated genes of ExPEC.

Source: Journal of Clinical Microbiology

DOI: 10.1128/jcm.39.10.3712-3717.2001

Authors: James R. Johnson, Adam L. Stell

Institutions: University of Minnesota, Minneapolis VA Medical Center

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green