Work

Creation of Enantioselective Biocatalysts for Organic Chemistry by In Vitro Evolution

Year: 1997

Type: article

Abstract: Evolution in the test tube: With the help of the error-prone polymerase chain reaction as a method for random mutagenesis, an efficient gene-expression system, and a screening test for the rapid identification of enantioselective catalysts, it is possible to increase sequentially the ee value of an unselective lipase-catalyzed ester hydrolysis (see picture on the right).

Source: Angewandte Chemie International Edition in English

DOI: 10.1002/anie.199728301

Authors: Manfred T. Reetz, Albin Zonta, Klaus Schimossek, Karl‐Erich Jaeger, Klaus Liebeton

Institutions: Max-Planck-Institut für Kohlenforschung, Ruhr University Bochum

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2100103592

FWCI: 4.068

Cites: 41

Cited by: 385

Related to: 9