IL-2 production correlates with effector cell differentiation in HIV-specific CD8+ T cells.
Year: 2006
Type: article
Study design: Observational Study
Abstract: BACKGROUND: Diminished IL-2 production and lack of effector differentiation have been reported for HIV-specific T cells. In this study, we examined the prevalence of these phenomena using 8-color cytokine flow cytometry, and tested the hypothesis that these two findings were causally related. We analyzed cytokine profiles and memory/effector phenotypes of HIV-specific and CMV-specific T cells using short-term in vitro stimulation with HIV or CMV peptide pools. Nineteen HIV-positive subjects with progressive disease and twenty healthy, HIV-negative subjects were examined. RESULTS: Among HIV-infected subjects, there were significantly fewer CD8+ IL-2+ T cells responding to HIV compared to CMV, with no significant difference in CD4+ IL-2+ T cells. The majority of CMV-specific T cells in both HIV-negative and HIV-positive subjects appeared to be terminally differentiated effector cells (CD8+ CD27- CD28- CD45RA+ or CD8+ CD27- CD28- CD45RA-). In HIV-positive subjects, the most common phenotype of HIV-specific T cells was intermediate in differentiation (CD8+ CD27+ CD28- CD45RA-). These differences were statistically significant, both as absolute cell frequencies and as percentages. There was a significant correlation between the absolute number of HIV-specific CD8+ IL-2+ T cells and HIV-specific CD8+ CD27- CD28- CD45RA+ terminal effector cells. CONCLUSION: IL-2 production from antigen-specific CD8+ T cells correlates with effector cell differentiation of those cells.
Source: AIDS Research and Therapy
Language: English
Topic: HIV Research and Treatment
Subfield: Virology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,990
Funders: Burroughs Wellcome Fund, National Institutes of Health, Center for AIDS Research, University of Washington, University of California, San Francisco, National Institute of Mental Health, National Institute of Allergy and Infectious Diseases, National Center for Research Resources, Center for AIDS Prevention Studies, University of California, San Francisco
Awards: Treatment Intensification for Drug-Resistant HIV, Assessing Laboratory Correlates of Immune Protection, NIH Director's Pioneer Award (RMI), DYNAMICS OF HIV1 SPECIFIC CTL IN HIV1 INFECTION, G4350804880, General Clinical Research Center, Immunologic Control of Drug-resistant HIV-1, G7717753738, G7998845928, Center for AIDS Prevention Studies (CAPS)
OpenAlex ID: W2100202814