Work

Direct Profiling of Environmental Microbial Populations by Thermal Dissociation Analysis of Native rRNAs Hybridized to Oligonucleotide Microarrays

Year: 2003

Type: article

Abstract: Oligonucleotide microarrays were used to profile directly extracted rRNA from environmental microbial populations without PCR amplification. In our initial inspection of two distinct estuarine study sites, the hybridization patterns were reproducible and varied between estuarine sediments of differing salinities. The determination of a thermal dissociation curve (i.e., melting profile) for each probe-target duplex provided information on hybridization specificity, which is essential for confirming adequate discrimination between target and nontarget sequences.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.69.4.2377-2382.2003

Authors: Said El Fantroussi, Hidetoshi Urakawa, Anne E. Bernhard, John J. Kelly, Peter A. Noble, H. Smidt, G. M. Yershov, David A. Stahl

Institutions: University of Washington, Loyola University Chicago, Argonne National Laboratory

Language: English

Topic: Microbial Community Ecology and Physiology

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2101253259

FWCI: 18.67

Cites: 27

Cited by: 124

Related to: 10