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Annotating genomes with massive-scale RNA sequencing

Year: 2008
Type: article
Abstract: Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
DOI: 10.1186/gb-2008-9-12-r175
Authors: France Denoeud, Jean-Marc Aury, Corinne Da Silva, Benjamin Noel, Odile Rogier, Massimo Delledonne, Michele Morgante, Giorgio Valle, Patrick Wincker, Claude Scarpelli, Olivier Jaillon, François Artiguenave
Institutions: Centre National de la Recherche Scientifique, Commissariat à l'Énergie Atomique et aux Énergies Alternatives, Génomique Métabolique du Genoscope, Genoscope, Université d'Évry Val-d'Essonne, University of Verona, Istituto di Genomica Applicata, University of Padua
Language: English
Open Access status: gold
Estimated APC paid: $5,490
OpenAlex ID: W2101466802
FWCI: 8.012
Cites: 53
Cited by: 255
Related to: 10