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Mechanisms involved in malabsorption of dietary lipids

Year: 1998
Type: dissertation
Abstract: Background & Aim: The C-mixed triglyceride (C-MTG) breath test has become popular for the detection of impaired intestinal lipolysis as a cause for fat malabsorption. However, the diagnostic value has been questioned because the relation between the extent of fat malabsorption and the corresponding result of the C-MTG breath test has not been established. We characterized the C-MTG breath test in rats with variable degrees of fat malabsorption, achieved by feeding the lipase inhibitor orlistat. Methods: Rats were fed high fat chow (35 en% fat) to which orlistat was added in amounts of 0, 50, 200, and 800 mg kg chow for 5 days. Breath CO2 recovery was determined for 6 h after oral administration of C-MTG (13 mg kg BW). Total dietary fat absorption was measured by means of a 3-day fecal fat balance. Results: Upon orlistat administration, total fat absorption decreased in a dose-dependent way from 80.2 ± 2.2% to 32.8 ± 3.7% (mean ± SEM; 0 mg and 800 mg orlistat kg chow, respectively; P<0.001). Correspondingly, breath CO2 recovery from CMTG at 6 h decreased from 84.5 ± 7.8% to 42.0 ± 1.5% of the dose (P<0.001). The 6-h recovery of breath CO2 appeared highly correlated with total fat absorption for the different dosages of orlistat (r=0.88, P<0.001). However, in rats with fat absorption higher than 70%, the coefficient of variation of cumulative breath CO2 excretion was large (15%) compared with that of fat absorption (5%). Conclusion: The C-MTG breath test correlates significantly with the extent of fat malabsorption in a rat model of impaired intestinal lipolysis. However, the considerable interindividual variation of the C-MTG breath test does not support its application for diagnostic purposes in individual patients. The C-MTG breath test in rats fed orlistat
Language: English
Field: Medicine
Open Access status: green
OpenAlex ID: W2101811402
Cites: 217
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Related to: 10