Work

Rat CYP2D2, not 2D1, is functionally conserved with human CYP2D6 in endogenous morphine formation

Year: 2012

Type: article

Abstract: The assumption that CYP2D1 is the corresponding rat cytochrome to human CYP2D6 has been revisited using recombinant proteins in direct enzyme assays. CYP2D1 and 2D2 were incubated with known CYP2D6 substrates, the three morphine precursors thebaine, codeine and (R)-reticuline. Mass spectrometric analysis showed that rat CYP2D2, not 2D1, catalyzed the 3-O-demethylation reaction of thebaine and codeine. In addition, CYP2D2 incubated with (R)-reticuline generated four products corytuberine, pallidine, salutaridine and isoboldine while rat CYP2D1 was completely inactive. This intramolecular phenol-coupling reaction follows the same mechanism as observed for CYP2D6. Michaelis-Menten kinetic parameters revealed high catalytic efficiencies for rat CYP2D2. These findings suggest a critical evaluation of other commonly accepted, however untested, CYP2D1 substrates.

Source: FEBS Letters

DOI: 10.1016/j.febslet.2012.05.021

Authors: Nadja Grobe, Toni M. Kutchan, Meinhart H. Zenk

Institution: Donald Danforth Plant Science Center

Language: English

Topic: Pharmacogenetics and Drug Metabolism

Subfield: Pharmacology

Field: Pharmacology, Toxicology and Pharmaceutics

Domain: Life Sciences

Open Access status: bronze

Funders: Edward Mallinckrodt, Jr. Foundation, National Institutes of Health, National Institute on Drug Abuse

Award: Morphine in Mammals