Work

Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bands

Year: 1975

Type: article

Abstract: The cell envelope of Enterobacteriaceae consists of two membranes separated by a peptidoglycan layer. Methods have been developed to separate the cytoplasmic membrane from the outer membrane. The outer membrane of Escherichia coli contains 60% of the envelope protein. Using polyacrylamide gelelectrophoresis, Schnaitman found six protein bands in the outer membrane fraction, of which one band (mol. wt 44 000) accounted for 70% of the total outer membrane protein.

Source: FEBS Letters

DOI: 10.1016/0014-5793(75)80272-9

Authors: Ben Lugtenberg, Jos Meijers, Roel Peters, Peter van der Hoek, Loek van Alphen

Institution: Utrecht University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2101848104

FWCI: 24.01

Cites: 19

Cited by: 1,415

Related to: 10