Year: 2010
Type: article
Abstract: In vitro shoot multiplications were obtained successfully from shoot tips of Ziziphus spina-christi by placing explants into solidified medium (MS medium) supplemented with 0.01 mg/l NAA and 0.1 mg/l BA or 0.1 mg/l IAA and 1.0 mg/l kinetin. It was concluded that lower concentrations of all cytokinin studied were better for lateral bud proliferation and that BA and 2IP were better than kinetin in the production of lateral branches. No growth regulators were required for shoot growth and elongation. The shoots rooted best on MS medium supplemented with 1.0 mg/l IBA. Plantlet survival after transfer to soil was more than 90%. The shoot proliferation method described could be used for the mass clonal propagation of selected genotype cv Noaf variety. The variety is in a great demand due to its attractive fruit characteristics such as flavour, sweetness and fruit yield. Key words: Clonal propagation, cidir, shoot tip culture, Ziziphus spina-christi (L.) Desf.
Source: AFRICAN JOURNAL OF BIOTECHNOLOGY
DOI: 10.5897/ajb09.1674
Institution: King Saud University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W2102037273