Partial Purification and Characterization of Extracellular Proteases of Klebsiella ox ytoca and Its Inhibition by the Volatile Oil of Eugenia uni flora
Year: 2013
Type: article
Abstract: The low vulnerability of pathogenic microbes to antibiotics and other conventional drugs has led to the need for the discovery of alternative therapy against such organisms. In this study, the antibacterial activity of the volatile oil of Eugenia uniflora was evaluated using the extracellular protease (ECP) of Klebsiella oxytoca as a potential d rug target. The volatile oil of E.uniflora inhibited the growth of Klebsiella oxytoca with MIC and MBC values of ≤ 0.8%v/v and ≥ 0.95%v/v respectively. The optimu m pH of the ext racellular protease was 6.5, wh ile the optimu m temperature was 45℃. The activ ity of K.oxytoca extracellu lar protease was found to be reduced in the presence of zinc and mercury while relatively higher activities were observed in the presence of Potassium, Calciu m, magnesium and manganese. The extracellular protease was observed to have an unaltered Vmax of 1.0 × 10 3 µmol/ min while the Km was determined as 1.140mg/ ml and 0.685mg/ ml in presence and absence of the volatile oil as an inhib itor respectively. Th is suggests that the mode of inhibit ion by the volatile oil on the extracellu lar protease fro m K.oxytoca is competit ive. The findings fro m th is study suggest that the volatile oil derived fro m E.uniflora is a potential therapeutic agent against K.oxytoca.
Source: American Journal of Biochemistry
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W2102070278