Year: 2008
Type: article
Abstract: We stretched a DNA molecule using atomic force microscope and quantified the mechanical properties associated with B and S forms of double-stranded DNA (dsDNA), molten DNA, and single-stranded DNA (ssDNA). We also fit overdamped diffusion models to the AFM time series and used these models to extract additional kinetic information about the system. Our analysis provides additional evidence supporting the view that S-DNA is a stable intermediate encountered during dsDNA melting by mechanical force. In addition, we demonstrated that the estimated diffusion models can detect dynamical signatures of conformational degrees of freedom not directly observed in experiments.
Institutions: Rice University, National Chung Hsing University
Language: English
Subfield: Atomic and Molecular Physics, and Optics
Field: Physics and Astronomy
Domain: Physical Sciences
Open Access status: bronze
Awards: MRI:"Acquisition" of Rice Computational Research Cluster (RCRC), Vertical Integration of Research and Education in the Mathematical Sciences, ITR/AP: Collaborative Research: Model Reduction of Dynamical Systems for Real-time Control, Nonequilibrium, Single-Molecule Studies of Protein Unfolding, NANOBIOLOGY INSTITUTIONAL TRAINING GRANT(RMI)
OpenAlex ID: W2102172821