Diversity of Helicobacter pylori genotypes in Estonian patients with chronic inflammatory gastric diseases
Year: 2006
Type: dissertation
Study design: Observational Study
Abstract: Background.In patients with perforated peptic ulcer (PPU) the convergence between the high eradication rate of Helicobacter pylori infection and low rates of ulcer relapse after treatment has been associated with reinfection by non-virulent strains.The persistence of infection by virulent H. pylori strains and ulcer recurrence were evaluated in 33 patients with PPU one year after surgery and antimicrobial treatment.Methods.The histological evaluation and molecular detection of H. pylori cagA and ureA genes, vacA allelic types and the PCR-RFLP analyses of the glmM gene products from antral mucosa specimens initially, 2-5 months and 1 year after therapy were performed.Results.Density of H. pylori colonization was temporarily decreased (p<0.05)2-5 months after therapy.After one year the complete eradication was achieved only in 7 patients (23%) at histological examination and recurrent ulcers were revealed in 3/33 (9%) patients.The vacA s1a allelic type of cagA positive strains persisted in 19 (83%) non-eradicated 23 PPU patients with identical PCR-RFLP fingerprints in 8/9 (89%) of the patients.Conclusions.In PPU patients with low eradication rate of H. pylori after surgical and antimicrobial treatment the frequent recrudescence of the infection is mostly caused by the persisting virulent strains of the cagA and vacA s1a subtypes.In the oneyear follow-up period the recurrent ulceration may be postponed just by lowered colonization density of H. pylori after eradicative therapy.
Author: Helena Andreson
Language: English
Subfield: Gastroenterology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Funders: Eesti Teadusfondi, Tartu Ülikool
OpenAlex ID: W2102248071