Work

Imaging Lipid Metabolism in Live Caenorhabditis elegans Using Fingerprint Vibrations

Year: 2014

Type: article

Abstract: Quantitation of lipid storage, unsaturation, and oxidation in live C. elegans has been a long-standing obstacle. The combination of hyperspectral stimulated Raman scattering imaging and multivariate analysis in the fingerprint vibration region represents a platform that allows the quantitative mapping of fat distribution, degree of fat unsaturation, lipid oxidation, and cholesterol storage in vivo in the whole worm. Our results reveal for the first time that lysosome-related organelles in intestinal cells are sites for storage of cholesterol in C. elegans.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201406029

Authors: Ping Wang, Bin Liu, Delong Zhang, Micah Y. Belew, Heidi A. Tissenbaum, Ji‐Xin Cheng

Institutions: Purdue University West Lafayette, University of Massachusetts Chan Medical School

Language: English

Topic: Spectroscopy Techniques in Biomedical and Chemical Research

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute on Aging, National Institute of General Medical Sciences