Work

G Protein Signaling Events Are Activated at the Leading Edge of Chemotactic Cells

Year: 1998

Type: article

Abstract: Directional sensing by eukaryotic cells does not require polarization of chemoattractant receptors. The translocation of the PH domain-containing protein CRAC in D. discoideum to binding sites on the inner face of the plasma membrane reflects activation of the G protein-linked signaling system. Increments in chemoattractant elicit a uniform response around the cell periphery. Yet when cells are exposed to a gradient, the activation occurs selectively at the stimulated edge, even in immobilized cells. We propose that such localized activation, transmitted by the recruitment of cytosolic proteins, may be a general mechanism for gradient sensing by G protein-linked chemotactic systems including those involving chemotactic cytokines in leukocytes.

Source: Cell

DOI: 10.1016/s0092-8674(00)81784-5

Authors: Carole A. Parent, Brenda J. Blacklock, Wendy M. Froehlich, Douglas B. Murphy, Peter N. Devreotes

Institutions: Johns Hopkins University, Johns Hopkins Medicine

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $11,400

Funders: National Institutes of Health, National Institute of General Medical Sciences