Work

Mutation of sarA in Staphylococcus aureus Limits Biofilm Formation

Year: 2003

Type: article

Abstract: Mutation of sarA resulted in a reduced capacity to form a biofilm in six of the eight Staphylococcus aureus strains we tested (UAMS-1, UAMS-601, SA113, SC-01, S6C, and DB). The exceptions were Newman, which formed a poor biofilm under all conditions, and RN6390, which consistently formed a biofilm only after mutation of agr. Mutation of agr in other strains had little impact on biofilm formation. In every strain other than Newman, including RN6390, simultaneous mutation of sarA and agr resulted in a phenotype like that observed with the sarA mutants. Complementation studies using a sarA clone confirmed that the defect in biofilm formation was due to the sarA mutation.

Source: Infection and Immunity

DOI: 10.1128/iai.71.7.4206-4211.2003

Authors: Karen E. Beenken, Jon S. Blevins, Mark S. Smeltzer

Institution: University of Arkansas for Medical Sciences

Language: English

Topic: Bacterial biofilms and quorum sensing

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Award: sar-mediated regulation in Staphylococcus aureus