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Extending the Option of CMV-Specific T Cells from the CMV-Seronegative Donor

Year: 2014
Type: article
Abstract: Adoptive transfer of CMVpp65-specific T-cells(cCTL) from CMV-seropositive(CMVpos) donors effectively restores antiviral-immunity after stem cell transplantation. However, the naïve T-cells in cord blood(CB) and adult CMV-seronegative(CMVneg) donors require different culture systems to generate cCTL. With a novel GMP-applicable protocol we have reliably generated cCTL from CB and found that 15/15 CB T-cell lines recognized atypical epitopes of pp65. We then expanded cCTL from CMVneg donors in a GMP-applicable way by isolating naive T-cells and stimulating them with pp65-Pepmix-pulsed antigen-presenting cells supplemented with IL2,IL7,IL12, and IL15.
DOI: 10.1016/j.bbmt.2013.12.200
Authors: Patrick J. Hanley, Sarah Nikiforow, Jan Melenhorst, Phillip Scheinberg, Barbara Savoldo, Gianpietro Dotti, Cliona M. Rooney, Helen E. Heslop, Elizabeth J. Shpall, A. John Barrett, Catherine M. Bollard
Institutions: Children's National, Dana-Farber Cancer Institute, National Institutes of Health, National Heart, Lung, and Blood Institute, Beneficência Portuguesa de São Paulo, Texas Children's Hospital, Baylor College of Medicine, Houston Methodist, The University of Texas MD Anderson Cancer Center
Language: English
Subfield: Epidemiology
Field: Medicine
Open Access status: hybrid
Estimated APC paid: $3,650
OpenAlex ID: W2102591618
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