Work

A 16S rRNA-targeted Probe for Detection of Lactobacilli and Enterococci in Faecal Samples by FluorescentIn SituHybridization

Year: 1999

Type: article

Abstract: A group-specific 16S rRNA-targeted oligonucleotide probe S-G-Lab-0158-a-A20 (Lab158) was designed and validated to quantify species of the phylogenetic group lactobacilli-enterococci. The Lab158 probe detects nearly all species of the genera Lactobacillus, Enterococcus, Pediococcus, Weissella, Vagococcus, Leuconostoc and Oenococcus. The specificity of the probe was tested on various species of the target group and on a range of common intestinal bacteria. For these experiments, procedures to permeabilize these groups of Gram-positive bacteria were optimized and fluorescent in situ hybridization (FISH) conditions for maximum specificity were determined. In addition, we showed that it is possible to distinguish the predominant gut-enterococci i.e. E. faecalis among the Lab158 probe-positive cells with the E. faecalis

Source: Microbial Ecology in Health and Disease

DOI: 10.3402/mehd.v11i1.7876

Authors: Hermie J. M. Harmsen, Peter Elfferich, Frits Schut, Gjalt W. Welling

Institution: University of Groningen

Language: English

Topic: Gut microbiota and health

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $1,725

Funder: Nederlandse Organisatie voor Wetenschappelijk Onderzoek