Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Chick Pea (Cicer arietinum L.) Var. Co - 4
Year: 2015
Type: article
Abstract: The present study was conducted in order to determine the effect of gamma rays and EMS on seed germination, Seedling height and root length in chick pea to identify the lethal dose (LD50). In this regard, the healthy seeds of chick pea was subjected to different doses/concentrations of gamma rays (20, 30, 40, 50 and 60kR) and EMS (10, 20, 30, 40 and 50mM) for inducing mutation. The effect of gamma rays and EMS was determined by measuring the seed germination, seedling height and root length under the conditions of the M1 generation. The results shows that, the seed germination, seedling height and root length were significantly decreased with increasing doses/concentrations. The LD50 values were observed based on the growth reduction of seedlings after treatments with mutagen. The effective doses/concentrations which caused 50% growth reduction were observed in 40kR in gamma rays and 30mM in EMS.
DOI: 10.56431/p-p7t41h
Authors: S. Umavathi, L. Mullainathan
Institution: Annamalai University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W2102876094