Work

Chemical Synthesis of Proteins

Year: 2005

Type: review

Abstract: Proteins have become accessible targets for chemical synthesis. The basic strategy is to use native chemical ligation, Staudinger ligation, or other orthogonal chemical reactions to couple synthetic peptides. The ligation reactions are compatible with a variety of solvents and proceed in solution or on a solid support. Chemical synthesis enables a level of control on protein composition that greatly exceeds that attainable with ribosome-mediated biosynthesis. Accordingly, the chemical synthesis of proteins is providing previously unattainable insight into the structure and function of proteins.

Source: Annual Review of Biophysics and Biomolecular Structure

DOI: 10.1146/annurev.biophys.34.040204.144700

Authors: Bradley L. Nilsson, Matthew B. Soellner, Ronald T. Raines

Institution: University of Wisconsin–Madison

Language: English

Topic: Chemical Synthesis and Analysis

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: Simons Foundation, National Institutes of Health, National Institute of General Medical Sciences

Award: Protein Chemistry