The Effects of Interleukin-1βin Tumor Necrosis Factor-α-Induced Acute Pulmonary Inflammation in Mice
Year: 2009
Type: article
Abstract: We determined the role of interleukin-1beta (IL-1beta) signaling on tumor necrosis factor alpha-induced (TNF-alpha) lung neutrophil influx as well as neutrophil chemoattractant macrophage inflammatory protein (MIP-2) and KC and soluble TNF-alpha receptor (TNFR) levels utilizing wildtype (WT), TNF receptor double knockout (TNFR1/TNFR2 KO), and IL-1beta KO mice after oropharyngeal instillation with TNF-alpha. A significant increase in neutrophil accumulation in bronchoalveolar lavage fluid (BALF) and lung interstitium was detected in the WT mice six hours after TNF-alpha exposure. This correlated with an increase in BALF MIP-2. In contrast, BALF neutrophil numbers were not increased by TNF-alpha treatment of IL-1beta KOs, correlating with a failure to induce BALF MIP-2 and a trend toward increased BALF soluble TNFR1. TNF-alpha-instillation increased lavage and serum KC and soluble TNFR2 irrespective of IL-1beta expression. These results suggest IL-1beta contributes, in part, to TNF-alpha-mediated, chemokine release, and neutrophil recruitment to the lung, potentially associated with altered soluble TNFR1 release into the BALF.
Source: Mediators of Inflammation
DOI: 10.1155/2009/958658
Institutions: University of Rochester Medicine, University of Rochester
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,040
Awards: G1523888516, G1588725948, Cell Specific Responses to TNF-alpha in Lung Fibrosis, Center for Biophysical Assessment and Risk Management, G1862412751, G2156078568, G2189277278, G2218005389, G2503229775, G257213359, G2915513768, G469873995, G4806922460, G4925750119, G4972920980, G6056225962, G8074153786, G9000020184, G9000218636, Environmental Agents as Modulators of Disease Processes, Training in Environmental Toxicology
OpenAlex ID: W2103077094