Work

In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules

Year: 1997

Type: article

Abstract: Using systematic evolution of ligands by exponential enrichment (SELEX), an RNA molecule was isolated that displays a 1,000-fold higher affinity for guanosine residues that carry an N-7 methyl group than for nonmethylated guanosine residues. The methylated guanosine residue closely resembles the 5' terminal cap structure present on all eukaryotic mRNA molecules. The cap-binding RNA specifically inhibited the translation of capped but not uncapped mRNA molecules in cell-free lysates prepared from either human HeLa cells or from Saccharomyces cerevisiae. These findings indicate that the cap-binding RNA will also be useful in studies of other cap-dependent processes such as pre-mRNA splicing and nucleocytoplasmic mRNA transport.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.94.16.8521

Authors: Aurelia A. Haller, Peter Sarnow

Institutions: Stanford Medicine, Stanford University, University of California, Santa Cruz

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green