Work

Improvement of Folate Biosynthesis by Lactic Acid Bacteria Using Response Surface Methodology

Year: 2010

Type: article

Abstract: Lactic acid bacteria (Lactococcus lactis NZ9000, Lactococcus lactis MG1363, Lactobacillus plantarum I-UL4 and Lactobacillus johnsonii DSM 20553) have been screened for their ability to produce folate intracellularly and/or extracellularly.L. plantarum I-UL4 was shown to be superior producer of folate compared to other strains.Statistically based experimental designs were used to optimize the medium formulation for the growth of L. plantarum I-UL4 and folate biosynthesis.The optimal values of important factors were determined by response surface methodology (RSM).The effects of carbon sources, nitrogen sources and para-aminobenzoic acid (PABA) concentrations on folate biosynthesis were determined prior to RSM study.The biosynthesis of folate by L. plantarum I-UL4 increased from 36.36 to 60.39 mg/L using the optimized medium formulation compared to the selective Man de Rogosa Sharpe (MRS) medium.Conditions for the optimal growth of L. plantarum I-UL4 and folate biosynthesis as suggested by RSM were as follows: lactose 20 g/L, meat extract 16.57g/L and PABA 10 mM.

Source: Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia)

Authors: Norfarina Muhamad Nor, Hooi Ling Foo, Raha Abdul Rahim, Rosfarizan Mohamad

Institution: Universiti Putra Malaysia

Language: English

Topic: Probiotics and Fermented Foods

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

Funder: Ministério da Ciência, Tecnologia e Inovação

OpenAlex ID: W2103257183