Work

Molecular cloning of the Escherichia coli O75X adhesin

Year: 1987

Type: article

Abstract: The uropathogenic strain Escherichia coli IH11128 (O75:K5:H-) exhibits a mannose-resistant O75X adhesin. The genes encoding the O75X adhesin were cloned from a clinical strain and transferred to E. coli K-12 derivatives. The recombinant plasmids were found to express a 15-kilodalton fimbrial subunit protein, a fimbrialike extracellular structure, and mannose-resistant hemagglutination. An indirect immunofluorescence assay was used to study attachment of the clone and purified adhesin to frozen sections of human kidney. The clone bound selectively to the interstitial areas and notably to Bowman's capsule. The purified adhesin bound to the basement membrane of the tubules and to Bowman's capsule.

Source: Infection and Immunity

DOI: 10.1128/iai.55.12.3168-3173.1987

Authors: B Nowicki, J P Barrish, T Korhonen, R A Hull, S I Hull

Institutions: Texas Medical Center, Baylor College of Medicine

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases