Work

Differential assembly of Cdc45p and DNA polymerases at early and late origins of DNA replication

Year: 1999

Type: article

Abstract: Chromosomes are replicated in characteristic, temporal patterns during S phase. We have compared the timing of association of replication proteins at early- and late-replicating origins of replication. Minichromosome maintenance proteins assemble simultaneously at early- and late-replicating origins. In contrast, Cdc45p association with late origins is delayed relative to early origins. DNA polymerase alpha association is similarly delayed at late origins and requires Cdc45p function. Activation of the S phase checkpoint inhibits association of Cdc45p with late-firing origins. These studies suggest that Cdc45p is poised to serve as a key regulatory target for both the temporal and checkpoint-mediated regulation of replication origins.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.96.16.9130

Authors: Oscar M. Aparicio, Anita M. Stout, Stephen P. Bell

Institution: Massachusetts Institute of Technology

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: American Cancer Society, National Institutes of Health, National Institute of General Medical Sciences

Award: Initiation of DNA Replication of Yeast Chromosomes