Application of Collagen Scaffolds for Hepatic Stem Like Cell Transplantation
Year: 2004
Type: article
Abstract: Tissue engineering using somatic stem cells is a new and exciting technique which has the potential to facilitate tissue reconstruction. Recent studies on tissue engineering have indicated advantages in using collagen matrices to restitute the structure of an organ. Here we have demonstrated the efficacy of transplantation of hepatic stem-like (HSL) cells, a previously established liver epithelial cell line with potential for differentiation, using type I collagen sponge. To this end, HSL cells were cultured and transplanted into Nagase's analbuminemic rat with spongy type I collagen matrices. Consequently, HSL was able to express both albumin and TAT after the culture in collagen sponge. In addition, the levels of serum albumin in recipient rats were found to increase up to 1.5 fold relative to controls after transplantation. These findings suggest that HSL cells are able to differentiate into functional hepatocytes, and that biodegradable collagen sponge may enhance hepatic differentiation of HSL cells by providing an appropriate microenvironment.
Authors: Yasuharu Ueno, Kunihiko Terada, Hirokazu Nagai, Go Watanabe, Kiyoshi Ishikawa, Kiwamu Yoshikawa, Yasuhiro Hirayama, Erika Endo, Takashi Kameda, Yang Liu, Toshihiro Sugiyama, Akita J Med
Language: Japanese
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2103663057