Work

Targeted Chromosomal Knockouts in Mycoplasma pneumoniae

Year: 2010

Type: article

Abstract: Most gene knockouts in mycoplasmas are achieved through labor-intensive transposon mutagenesis. Here, we describe a method for making targeted deletions in Mycoplasma pneumoniae by use of homologous recombination. In this method, M. pneumoniae is transformed with a plasmid carrying an antibiotic resistance marker flanked by 1-kb regions surrounding the target gene. Following selection for the antibiotic resistance, colonies are screened for double crossovers which indicate complete deletion of the target open reading frame.

Source: Applied and Environmental Microbiology

DOI: 10.1128/aem.00024-10

Authors: Radha Krishnakumar, Nacyra Assad-Garcia, Gwynedd A. Benders, Quang Phan, Michael G. Montague, John I. Glass

Institution: J. Craig Venter Institute

Language: English

Topic: Microbial infections and disease research

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2103921679

FWCI: 2.939

Cites: 30

Cited by: 33

Related to: 10