Work

MicroRNA-184-mediated inhibition of tumour growth in an orthotopic murine model of neuroblastoma.

Year: 2010

Type: article

Abstract: BACKGROUND: Neuroblastoma is a paediatric cancer which originates from precursor cells of the sympathetic nervous system. Previous studies have shown that miR-184 expression has anti-proliferative effects in neuroblastoma cells grown in culture. Therefore, it was of interest to evaluate this effect in vivo. MATERIALS AND METHODS: Neuroblastoma cells overexpressing miR-184 were injected retroperitoneally into CB17-SCID mice and tumour burden was assessed by measuring bioluminescence. Overall survival was also evaluated. RESULTS: Ectopic overexpression of miR-184 in neuroblastoma cell lines is anti-proliferative. In addition, overexpression of miR-184 led to a significant reduction in tumour growth relative to negative control-treated cohorts in a xenograft model of neuroblastoma. CONCLUSION: This study demonstrated for the first time that miR-184 significantly reduces tumour growth and increases overall survival in an orthotopic murine model of neuroblastoma through assessment of tumour growth and moribundity relative to control miRNA-treated cohorts.

Source: PubMed

Authors: Amanda Tivnan, Niamh H Foley, Lorraine Tracey, Andrew M Davidoff, Raymond L Stallings

Institution: Royal College of Surgeons in Ireland

Language: English

Topic: MicroRNA in disease regulation

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

Awards: Studies of Childhood Solid Tumors, MiRNA Expression in Neuroblastoma, Cancer Center Support Grant (CCSG)