Work

Determination of cholesterol using o-phthalaldehyde

Year: 1973

Type: article

Abstract: A simple, rapid method for the determination of cholesterol in plasma and tissue using o-phthalaldehyde is presented. Comparison of this method with the FeCl(3) method gave identical results. However, the o-phthalaldehyde determination is three times more sensitive than the FeCl(3) determination (molar extinction coefficients of 11,610 and 33,440 for FeCl(3) and o-phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation.

Source: Journal of Lipid Research

DOI: 10.1016/s0022-2275(20)36896-6

Authors: L.L. Rudel, M.D. Morris

Institutions: University of Toronto, University of Arkansas Medical Center

Language: English

Topic: Cholesterol and Lipid Metabolism

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W2104010651

FWCI: 8.545

Cites: 3

Cited by: 1,050

Related to: 10