Work

Enhanced production of flavonoids by methyl jasmonate elicitation in cell suspension culture of Hypericum perforatum

Year: 2015

Type: article

Abstract: Background Flavonoids of Hypericum perforatum are important secondary metabolites which have been widely utilized in medicine for a range of purposes. The use of methyl jasmonate (MeJA) elicitation for the enhancement of flavonoid production in cell suspension culture of H. perforatum would be an efficient alternative method for the flavonoid production. Results MeJA influenced the cells growth and flavonoid production. The optimal elicitation strategy was treatment of the cell cultures with 100 μmol/L MeJA on day 15, which resulted in the highest flavonoid production (280 mg/L) and 2.7 times of control cultures. The activities of catalase (CAT) were inhibited after MeJA treatment in the cell cultures, while the activities of phenylalanine ammonia lyase (PAL) increased, which led to the enhancement of flavonoid production. Conclusion MeJA elicitation is a useful method for the enhancement of flavonoid production in cell suspension culture of H. perforatum.

Source: Bioresources and Bioprocessing

DOI: 10.1186/s40643-014-0033-5

Authors: Jing Wang, Jie Qian, Lingyun Yao, Yanhua Lu

Institutions: East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Tongji University

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,890

Funders: State Key Laboratory of Bioreactor Engineering, Fundamental Research Funds for the Central Universities