Work

Plasma Membrane Ca2+ ATPase 4 Is Required for Sperm Motility and Male Fertility

Year: 2004

Type: article

Abstract: Calcium and Ca(2+)-dependent signals play a crucial role in sperm motility and mammalian fertilization, but the molecules and mechanisms underlying these Ca(2+)-dependent pathways are incompletely understood. Here we show that homozygous male mice with a targeted gene deletion of isoform 4 of the plasma membrane calcium/calmodulin-dependent calcium ATPase (PMCA), which is highly enriched in the sperm tail, are infertile due to severely impaired sperm motility. Furthermore, the PMCA inhibitor 5-(and-6)-carboxyeosin diacetate succinimidyl ester reduced sperm motility in wild-type animals, thus mimicking the effects of PMCA4 deficiency on sperm motility and supporting the hypothesis of a pivotal role of the PMCA4 on the regulation of sperm function and intracellular Ca(2+) levels.

Source: Journal of Biological Chemistry

DOI: 10.1074/jbc.m312599200

Authors: Kai Schuh, Elizabeth J. Cartwright, Eriks Jankevics, Karin Bundschu, Jürgen Liebermann, Judith C. Williams, Angel L. Armesilla, Michael Emerson, Delvac Oceandy, Klaus-Peter Knobeloch, Ludwig Neyses

Institutions: University of Würzburg, University of Manchester, University of Latvia, Leibniz-Forschungsinstitut für Molekulare Pharmakologie, University of Luxembourg

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W2104174115

FWCI: 9.721

Cites: 38

Cited by: 274

Related to: 9