DOPAMINE RECEPTOR SUBTYPES, IP3, cAMP AND cGMP FUNCTIONAL REGULATION IN PARKINSONISM INDUCED BY UNILATERAL INFUSION OF ROTENONE IN RATS: RECOVERY WITH BONE MARROW CELLS, SEROTONIN AND GABA SUPPLEMENTATION
Year: 2011
Type: dissertation
Abstract: In the present study, the effects of 5-HT, GABA and Bone Marrow Cells\ninfused intranigrally to substantia nigra individually and in combinations on\nunilateral rotenone infused Parkinsonism induced rats. Scatchard analysis of DA,\nDA D1 and D2 receptors in the corpus striatum, cerebral cortex, cerebellum, brain\nstem and hippocampus showed a significant increase in the Brain regions of\nrotenone infused rat compared to control. Real Time PCR amplification of DA D1,\nD2, Bax and ubiquitin carboxy-terminal hydrolase were up regulated in the brain\nregions of rotenone infused rats compared to control. Gene expression studies of\n -Synuclien, cGMP and Cyclic AMP response element-binding protein showed a\nsignificant down regulation in Rotenone infused rats compared to control.\nBehavioural studies were carried out to confirm the biochemical and molecular\nstudies.Our study demonstrated that BMC administration alone cannot reverse the\nabove said molecular changes occurring in PD rat. 5-HT and GABA acting\nthrough their specific receptors in combination with bone marrow cells play a\ncrucial role in the functional recovery of PD rats. 5-HT, GABA and Bone marrow\ncells treated PD rats showed significant reversal to control in DA receptor binding\nand gene expression. 5-HT and GABA have co-mitogenic property. Proliferation\nand differentiation of cells re-establishing the connections in Parkinson's disease facilitates the functional recovery. Thus, it is evident that 5-HT and GABA along\nwith BMC to rotenone infused rats renders protection against oxidative, related\nmotor and cognitive deficits which makes them clinically significant for cellbased\ntherapy. The BMC transformed to neurons when co-transplanted\nwith 5-HT and GABA which was confirmed with PKH2GL and nestin.\nThese newly formed neurons have functional significance in the therapeutic\nrecovery of Parkinson’s disease.
Author: Jes Paul
Language: English
Subfield: Clinical Neurology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W2104512859