Role of BacA in Lipopolysaccharide Synthesis, Peptide Transport, and Nodulation by Rhizobium sp. Strain NGR234
Year: 2011
Type: article
Abstract: BacA of Sinorhizobium meliloti plays an essential role in the establishment of nitrogen-fixing symbioses with Medicago plants, where it is involved in peptide import and in the addition of very-long-chain fatty acids (VLCFA) to lipid A of lipopolysaccharide (LPS). We investigated the role of BacA in Rhizobium species strain NGR234 by mutating the bacA gene. In the NGR234 bacA mutant, peptide import was impaired, but no effect on VLCFA addition was observed. More importantly, the symbiotic ability of the mutant was comparable to that of the wild type for a variety of legume species. Concurrently, an acpXL mutant of NGR234 was created and assayed. In rhizobia, AcpXL is a dedicated acyl carrier protein necessary for the addition of VLCFA to lipid A. LPS extracted from the NGR234 mutant lacked VLCFA, and this mutant was severely impaired in the ability to form functional nodules with the majority of legumes tested. Our work demonstrates the importance of VLCFA in the NGR234-legume symbiosis and also shows that the necessity of BacA for bacteroid differentiation is restricted to specific legume-Rhizobium interactions.
Source: Journal of Bacteriology
DOI: 10.1128/jb.01260-10
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
Awards: G1148400999, G115765347, G222768804, Fine Tuning of Nodulation by Complementary Rhizobial Signals, MIT Center for Environmental Health Sciences, Exemplarische Darstellung der Werke und des Lebens von Marcus Lombardus, eines Grenzgängers zwischen jüdischer und christlicher Religion und Gesell-, Rhizobium-plant symbioses require the precise timing and recognition of rhizobial secondary symbiotic signals, G8832621192, G9000300833
OpenAlex ID: W2104647883