Work

Characterization of Murine A-raf, a New Oncogene Related to the v-raf Oncogene

Year: 1986

Type: article

Abstract: A 1.6-kilobase cDNA (A-raf) has been isolated from a murine spleen cDNA library which encodes part of a protein related to the raf oncogene. Its amino acid sequence has 85% homology to raf in a central portion of 100 amino acids. In contrast to raf, A-raf shows a highly restricted tissue distribution of expression, with highest levels observed in epididymis, followed by intestine. When incorporated into a retrovirus, the resulting gag-A-raf fusion gene causes transformation in vitro and induces tumors in newborn mice. Thus, A-raf represents a new proto-oncogene. Transformation by A-raf is independent of ras gene function, as is the case for raf and mos but not other oncogenes.

Source: Molecular and Cellular Biology

DOI: 10.1128/mcb.6.7.2655

Authors: M Huleihel, M Goldsborough, J Cleveland, M Gunnell, T Bonner, U R Rapp

Institutions: Frederick National Laboratory for Cancer Research, National Cancer Institute, National Institute of Mental Health

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: U.S. Department of Health and Human Services, National Institutes of Health, National Cancer Institute