Work

Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family.

Year: 1989

Type: article

Abstract: Recently, hematopoietic stem cells were purified to homogeneity from mouse bone marrow. The protein structure of Sca-1, the cell surface antigen used in the isolation of hematopoietic stem cells, is described here. It is shown that the Sca-1 antigen is a member of the Ly-6 antigen family. The anti-Sca-1 antibody was used in immunohistochemistry experiments to define the structures in several tissues that had previously been shown to contain Ly-6 antigens. In thymus, spleen, and kidney, specific staining of parenchymal cells can be demonstrated, whereas only vasculature reacts with anti-Sca-1 in brain, heart, and liver and possibly in lung.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.86.12.4634

Authors: M van de Rijn, S Heimfeld, G J Spangrude, I L Weissman

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Hematopoietic Stem Cell Transplantation

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases