Work

Ferric iron uptake in Erwinia chrysanthemi mediated by chrysobactin and related catechol-type compounds

Year: 1992

Type: article

Abstract: Erwinia chrysanthemi 3937 possesses a saturable, high-affinity transport system for the ferric complex of its native siderophore chrysobactin, [N-alpha-(2,3-dihydroxybenzoyl)-D-lysyl-L-serine]. Uptake of 55Fe-labeled chrysobactin was completely inhibited by respiratory poison or low temperature and was significantly reduced in rich medium. The kinetics of chrysobactin-mediated iron transport were determined to have apparent Km and Vmax values of about 30 nM and of 90 pmol/mg.min, respectively. Isomers of chrysobactin and analogs with progressively shorter side chains mediated ferric iron transport as efficiently as the native siderophore, which indicates that the chrysobactin receptor primarily recognizes the catechol-iron center. Free ligand in excess only moderately reduced the accumulation of 55Fe. Chrysobactin may therefore be regarded as a true siderophore for E. chrysanthemi.

Source: Journal of Bacteriology

DOI: 10.1128/jb.174.14.4783-4789.1992

Authors: M Persmark, D Expert, J B Neilands

Institution: University of California, Berkeley

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, U.S. Public Health Service, National Institute of Allergy and Infectious Diseases

Awards: MICROBIAL IRON METABOLISM, G54774419