Natural and artificial production of protoplasts from heterothallic and homothallic Closterium
Year: 1983
Type: article
Abstract: Protoplasts were produced from the vegetative cells of Closterium peracerosum strigosum-littorale (heterothallic) and C. acerosum (homothallic) by the treatment with 4% cellulase Onozuka R-I0 and 0.5% Macerozyme R-I0. The yields of the protoplasts from KAS-4-30 strain (mating type-plus) were higher than those from KAS-4-29 (mating type-minus) of the former species. In C. acerosum, it was only 8% .• The protoplast productions from the mating type-plus and -minus cells were inhibited with more than 0.55 and 0.7 M sorbitol, respectively. During the process, chloroplasts were swollen and turned into spiral form in the protoplasts. Such behavior of the chloroplasts was not observed during the natural mating process of the heterothallic and homothallic strains and in vegetative cells of the homothallic strain. Cell wall was regenerated in the protoplasts of homothallic strain within 5 hr after removal of the lytic enzymes, but not in the heterothallic strains so far.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2105505258