Work

RNA Dynamics by Design: Biasing Ensembles Towards the Ligand‐Bound State

Year: 2010

Type: article

Abstract: Window of opportunity: Using a single A-U to G-C mutation, the local and global dynamic characteristics of the transactivation response element (TAR) RNA was rationally altered over timescales extending up to milliseconds. This procedure allows it to mimic its bound state with the ligand argininamide (ARG). The mutant binds ARG with slightly enhanced affinity using a conformation indistinguishable from the wild-type sequence.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201000814

Authors: Andrew C. Stelzer, Jeremy D. Kratz, Qi Zhang, Hashim M. Al‐Hashimi

Institution: University of Michigan

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Award: Targeting HIV-1 Regulatory RNA Elements